Anti-IRAK2 Antibody

Rabbit Polyclonal Antibody specific to IRAK2

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

IRAK-2

Gene Name

IRAK2

Gene ID

3656

NCBI Gene ID

UniProt

O43187

Accession Number

NP_001561

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 546- 564 of human IRAK2.

Target Antigen

Interleukin-1 receptor-associated kinase-like 2

Target

IRAK2

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. {PubMed:10383454, PubMed:9374458}.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution. <br><br>Positive control: Whole cell lysate from HeLa cells or K562 cells. No cross- reactivity with IRAK.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes full-length human IRAK2 (65 kD).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

The pro-inflammatory cytokine IL-1 induces cellular responses through two subunits of its receptor, IL-1 receptor 1 (IL-1R1) and IL- 1 receptor accessory protein (IL-1RacP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappaB, a key transcription factor that mediates inflammatory and immune respones. A novel member of the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 associate with the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappaB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kappaB activation. Members of the IRAK/Pelle family play a central role in IL-1R and TLR- mediated inflammatory responses. IRAK2 is expressed in a variety of human tissues.