Anti-BRD2 Antibody

Goat Polyclonal Antibody specific to BRD2

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

O27.1.1, Really interesting new gene 3 protein

Gene Name

BRD2

NCBI Gene ID

UniProt

P25440

Accession Number

NP_001106653

Cellular Locus

Nucleus

Host

Goat

Reactivity

Human

Immunogen

Synthetic peptide representing a portion of the protein between the putative nuclear localization signal and the ET domain

Target Antigen

Bromodomain-containing protein 2

Target

BRD2

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

WB, IP

Field of Research

Enzymes

Purification Method

Purified by immunoaffinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Tris

Function

May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly. {PubMed:18406326}.

Additionnal Information

Immunoblotting: 0.025 - 0.1 ug/mL.Detection of human BRD2 in RIPA extract (10ug) of HeLa cells untreated (-) or treated (+) with calf intestinal phosphatase with #13020 at 0.05ug/mL.

Storage Conditions

This product is stable at 2 -8°C for 1 year.

Specificity

This antibody reacts with human BRD2.The specific epitope is in the region between aa 550 and 600 of human BRD2.Based on 100% sequence identity, this antibody is predicted to react with Bovine, Rhesus Monkey, Gorilla, White-tufted-ear marmoset, Crab-eating macaque and Northern white-cheeked gibbon.Antibody is affinity-purified with an epitope specific to BRD2 immobilized on solid support.

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Buffer pH

pH 7.8

Target Background

BRD2 (bromodomain-containing 2) is a nuclear mitogen-activated kinase that plays a role in transcription of cell-cycle- regulated genes by functioning as an adapter protein that recruits E2F and chromatin remodeling factors to E2F response elements.