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Recombinant Human Noggin

Product Specifications

Product Name Alternative

Noggin, NOG

UniProt

Q13253

Expression Region

28-232

Host

Mammalian Cells

Tag

No tag

Applications

Western Blot, ELISA

Field of Research

Stem Cells, Developmental biology

Endotoxin

< 0.01 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 10-100 ng/ml in the presence of 300 ng/mL of Recombinant Human BMP‑4.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

25-33 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, 2 mM EDTA, pH 7.4.

Organism Species

Human

Available Sizes

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