Recombinant Human Noggin
Product Specifications
Product Name Alternative
Noggin, NOG
UniProt
Q13253
Expression Region
28-232
Host
Mammalian Cells
Tag
No tag
Applications
Western Blot, ELISA
Field of Research
Stem Cells, Developmental biology
Endotoxin
< 0.01 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 10-100 ng/ml in the presence of 300 ng/mL of Recombinant Human BMP‑4.
Form
Lyophilized powder
Reconstitution
Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Molecular Weight
25-33 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.
Formulation
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, 2 mM EDTA, pH 7.4.
Organism Species
Human
Available Sizes
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