CheKine™ Mirco Sucrose Phosphorylase (SP) Activity Assay Kit

SucrosePhosphorylase (SP, EC2.4.1.7) is mainly present in microorganisms and plants and belongs to the glycosylhydrolase 13 family. It is an enzyme that catalyzes the transfer of glucoside bonds and can catalyze the synthesis of 1-phospho-glucose from sucrose and inorganic phosphate. This enzyme mainly uses sucrose and glucose 1-phosphate as donors, and many substances such as polyhydroxyl sugars, sugar alcohols, phenolic hydroxyl groups, carboxyl groups as receptors to catalyze the synthesis of various glycosides. CheKine™ Micro Sucrose Phosphorylase (SP) Activity Assay Kit can detect plant tissues, fungus samples. In this kit, SP can catalyze sucrose to produce glucose 1-phosphate, which is modified to glucose 6-phosphate under the catalysis of glucose phosphomutase, and reduce NADP+ to generate NADPH under the action of glucose 6-phosphate dehydrogenase, resulting in an increase in the light absorption value of 340nm. The SP activity was reflected by the increase rate of 340nm absorbance.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.