AM1-43 Nerve Terminal Staining Kit IIB

Nerve terminal dyes have highly hydrophilic, cationically charged head groups at one end with lipophilic tails at the other end. They are virtually non-fluorescent in aqueous solution, but become intensely fluorescent in membranes. Following nerve stimulation, the dye molecules are internalized in newly formed endocytic vesicles. During exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescence signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity. Nerve Terminal Staining Kit IIB contains AM1-43 nerve terminal dye and SCAS (4-Sulfonato calix[8]arene, sodium salt) background reducing agent which dramatically lowers background fluorescence as soon as it is added to the preparation without the need for repeated wash steps.

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.