SynaptoGreenTM C18 (equivalent to FM®3-25) Nerve Terminal Dye

Nerve terminal dyes have highly hydrophilic, cationically charged head groups at one end with lipophilic tails at the other end. They are virtually non-fluorescent in aqueous solution, but become intensely fluorescent in membranes. Following nerve stimulation, the dye molecules are internalized in newly formed endocytic vesicles. During exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescence signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity. SynaptoGreenTM C18 is a widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. SynaptoGreenTM C18 (equivalent to FM®3-25) is a membrane stain with a chemical structure similar to other nerve terminal dyes, except that it has the longest lipophilic tail (C18) . Because of its large size, it is used as a control for ion channel permeability of nerve terminal dyes.

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.