Mag-Fura-2, AM Ester Fluorescent Magnesium Indicator
Our Mag-Fura-2 AM Ester is a fluorescent magnesium and calcium indicator that can be loaded into cells via incubation. Calcium and magnesium imaging are widely used to measure neuronal activity.
Product Specifications
Background
Mag-Fura-2, AM ester is a membrane-permeant form of Mag-Fura-2. The indicator is loaded into cells via simple incubation. Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a Kd of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca2+ but with a significantly higher Kd than Fura-2 for Ca2+. An important application of Mag-Fura-2 is its use in detecting high, transient Ca2+ concentration during Ca2+ spikes.
Type
Dyes & Tracing Reagents
Applications
ICC, IHC, EM
Molecular Weight
723 Da
Shipping Conditions
Shipped at ambient temperature with desiccant; protected from light
Storage Conditions
Store at –20°C, with desiccant; protect from light.
Specificity
Membrane-permeant, ratiometric; can be loaded into cells via incubation.
Recommended Usage
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Physical Properties
Solid
Conjugate
Kd: 1.9 mM, Ex: 369 nm, Em: 511 nm (no Mg2+) ; Ex: 330 nm, Em: 491 nm (high Mg2+)
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