Fluo-3, AM Ester Fluorescent Calcium Indicator

Our Fluo-3, AM Ester is a non-ratiometric calcium indicator that increases in fluorescence intensity upon calcium binding. Calcium imaging is widely used to measure neuronal activity.

Product Specifications

Background

To study the roles of calcium, a key intracellular messenger in cells, it is essential to quantitatively monitor its concentration. The most widely used method of Ca2+ detection is the use of fluorescent Ca2+ indicators. Fluo-3, AM ester is a membrane-permeant form of Fluo-3 that can be loaded into cells via incubation. Fluo-3 AM ester itself does not bind Ca2+, but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.

Type

Dyes & Tracing Reagents

Applications

ICC, IHC, EM

Molecular Weight

1129.9 Da

Shipping Conditions

Shipped at ambient temperature with desiccant; protected from light

Storage Conditions

Store at –20°C, with desiccant; protect from light.

Specificity

Membrane-permeant, non-ratiometric; can be loaded into cells via incubation.

Recommended Usage

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.