Fluo-3, Pentapotassium Salt Fluorescent Calcium Indicator
Our Fluo-3, Pentapotassium Salt is a non-ratiometric calcium indicator that increases in fluorescence intensity upon calcium binding. Calcium imaging is widely used to measure neuronal activity.
Product Specifications
Background
To study the roles of calcium, a key intracellular messenger in cells, it is essential to quantitatively monitor its concentration. The most widely used method of Ca2+ detection is the use of fluorescent Ca2+ indicators. Fluo-3, pentapotassium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading. As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed.
Type
Dyes & Tracing Reagents
Applications
ICC, IHC, EM
Molecular Weight
960 Da
Shipping Conditions
Shipped at ambient temperature with desiccant; protected from light
Storage Conditions
Store at 2°C to 8°C, with desiccant; protect from light.
Specificity
Membrane-impermeant, non-ratiometric; can be loaded into cells via microinjection or scrape loading.
Recommended Usage
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Physical Properties
Solid
Conjugate
Kd: 450 nM, Ex: 506 nm, Em: 526 nm
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