Phaseolus Vulgaris Leucoagglutinin (PHA-L) Lectin, CF®680 Conjugate
Our Phaseolus Vulgaris Leucoagglutinin (PHA-L) conjugated to CF®680 is used to trace connections in the central nervous system. It's transported by neurons in an anterograde direction, preferentially labeling axons, dendrites, and terminal boutons.
Product Specifications
Background
Lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins. The presence and distribution of these targets vary between cell types and tissues. Phaseolus vulgaris (red kidney bean) agglutinin (phytohemagglutinin; PHA) is a family of lectins, each consisting of four subunits. There are two different types of subunits. The “E” (erythroagglutinin) subunit causes red cell agglutination, and the “L” (lymphocyte) subunit can trigger lymphocyte agglutination and mitogenic activity. PHA-L is used to trace connections in the central nervous system. It's transported by neurons in an anterograde direction, preferentially labeling axons, dendrites, and terminal boutons. Conjugated to CF®680.
Type
Dyes & Tracing Reagents
Applications
ICC, IHC, EM
Concentration
1 mg/mL
Buffer
10 mM HEPES pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2
Shipping Conditions
Shipped at ambient temperature; protected from light
Storage Conditions
Store at 2°C to 8°C, protect from light.
Specificity
Membrane impermeant; works in live/intact cells and cells fixed in formaldehyde or methanol.
Recommended Usage
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Physical Properties
Solution
Conjugate
CF®680 Ex: 681 nm, Em: 698 nm
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