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Phaseolus Vulgaris Leucoagglutinin (PHA-L) Lectin, CF®594 Conjugate

Our Phaseolus Vulgaris Leucoagglutinin (PHA-L) conjugated to CF®594 is used to trace connections in the central nervous system. It's transported by neurons in an anterograde direction, preferentially labeling axons, dendrites, and terminal boutons.

Product Specifications

Background

Lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins. The presence and distribution of these targets vary between cell types and tissues. Phaseolus vulgaris (red kidney bean) agglutinin (phytohemagglutinin; PHA) is a family of lectins, each consisting of four subunits. There are two different types of subunits. The “E” (erythroagglutinin) subunit causes red cell agglutination, and the “L” (lymphocyte) subunit can trigger lymphocyte agglutination and mitogenic activity. PHA-L is used to trace connections in the central nervous system. It's transported by neurons in an anterograde direction, preferentially labeling axons, dendrites, and terminal boutons. Conjugated to CF®594.

Type

Dyes & Tracing Reagents

Applications

ICC, IHC, EM

Concentration

1 mg/mL

Buffer

10 mM HEPES pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2

Shipping Conditions

Shipped at ambient temperature; protected from light

Storage Conditions

Store at 2°C to 8°C, protect from light.

Specificity

Membrane impermeant; works in live/intact cells and cells fixed in formaldehyde or methanol.

Recommended Usage

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Physical Properties

Solution

Conjugate

CF®594 Ex: 593 nm, Em: 614 nm

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