FL550 Antibody Labeling Kit

To perform multiplex analysis for immunofluorescence (IF) or immunohistochemistry, antibody conjugates are often used but are not always commercially available. As an alternative option, and to support their research, scientists often create their own conjugates through selecting carrier free antibodies and conjugation kits which allows for flexibility to tailor design a set of conjugates (correct combination of antibodies with fluors, enzymes or biotin) . The easy to use FL550 antibody labeling kits conjugates 50-100 μg of purified antibody (0.5-1.0 mg/mL) to FL550 in 10 minutes. Please note - for high labeling efficiency, the starting antibody concentration should be 0.5-1.1 mg/ml in buffer free of extraneous proteins such as BSA, serum or gelatin. Antibody formulations containing azide, trehalose or other sugars are acceptable. No tris buffer. Benefits of the kit include: Start to finish time is ~10 minutes and requires 15-30 seconds hands-on time. Antibody yield 95% to 10%. The kit is extremely easy to use - no prior conjugation experience is needed. Select from a diverse range of antibody labels. All components are included in each kit.

Antibody Preparation 1) Add 1/10 volume of 10X modifier directly to your antibody tube (i.e. for 100 μl of antibody, add 10 μl of modifier) . Labeling with FL550 2) Add antibody solution to the FL550 tube, mix thoroughly with pipette or vortex. Incubate in the dark at room temperature for 10 minutes. Final buffer step 3) After the 10-minute incubation is complete, add 90 μL final buffer diluent to FL550-labeled antibody, vortex, and it is now ready to use in your assay. Final antibody concentration is ~0.25-0.5 mg/mL, depending on starting concentration.