Anti-Epithelial Cell Adhesion Molecule (Ep-CAM) Antibody (BSA and Azide free)

Epithelial specific antigen is a transmembrane glycoprotein (40 kDa) also known as ESA or epithelial cellular adhesion molecule (Ep-CAM) . This protein is expressed on basolateral cell surface in most simple epithelia and vast majority of cancers. According to the “Cancer Stem Cells” (CSC) theory, tumors are not viewed as simple monoclonal expression of transformed cells, but rather as complex tissue where abnormal growth is driven by a minority pathological CSC pool that on one hand has acquired tumor related features such as uncontrolled growth and ability to form metasis and on the other hand maintains its inherent capacity to self renew and differentiate into a phenotypic ally heterogeneous, although aberrant progency. This antibody has been used recently to characterize the EpCAM high CD44+ versus EpCAM low/Cd44- indifferent cancer cells.

Our Anti-Epithelial Cell Adhesion Molecule mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone ESA 214. It detects human Epithelial Cell Adhesion Molecule, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and buffer exchanged into 1X PBS. Purified mAbs are >90% specific antibody.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.