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Anti-Cytokeratin Cocktail Antibody

Our Anti-Cytokeratins 1-6, 8, 10, 13-16, 18, 19 mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone Multiple clones. It detects human Cytokeratins 1-6, 8, 10, 13-16, 18, 19, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB, ELISA.

Product Specifications

Background

Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46) . This antibody recognizes cytokeratins CK1 (67), CK2 (65.5), CK3 (64), CK4 (59) CK5 (58), CK6 (56), CK8 (52.5), CK10 (56.5), CK13 (54) CK14 (50), CK15 (50), CK16 (48), CK18 (45), CK 19 (40)

Short Description

Our Anti-Cytokeratins 1-6, 8, 10, 13-16, 18, 19 mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone Multiple clones. It detects human Cytokeratins 1-6, 8, 10, 13-16, 18, 19, and is purified by protein a chromatography. It is great for use in IHC, ICC, WB, ELISA.

Gene Name

KRT1-6, 8, 10, 13-16, 18, 19

Host

Mouse

Reactivity

Human

Immunogen

Human epidermal keratin

Target

Cytokeratins 1-6, 8, 10, 13-16, 18, 19

Clonality

Monoclonal

Isotype

IgG1

Clone

Multiple clones

Type

Primary Antibody

Applications

IHC, ICC, WB, ELISA

Field of Research

Cytoskeletal Markers/Cytoskeletal Regulation

Concentration

1 mg/mL

Format

Purified by Protein A Chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

40-67 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:100 - 1:500

WB Dilution

1:1000 -1:5000

Specificity

No cross-reactivity reported

ICC Dilution

1:200 - 1:1000

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Quality Control

1

Conjugate

Unconjugated

RRID

AB_3095363

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