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Anti-Epithelial Membrane Antigen (EMA)

Our Anti-Epithelial Membrane Antigen (EMA) mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone EMA 201. It detects human Epithelial Membrane Antigen (EMA), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB, ELISA.

Product Specifications

Background

Epithelial membrane antigens are high molecular weight mucin-like glycoproteins. Antibodies to EMA have been shown to be useful as pan-epithelial markers for detection of early metastatic loci of carcinoma in the bone marrow or liver.

Short Description

Our Anti-Epithelial Membrane Antigen (EMA) mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone EMA 201. It detects human Epithelial Membrane Antigen (EMA), and is purified by protein a chromatography. It is great for use in IHC, ICC, WB, ELISA.

Synonyms

CD227, MUC1, CA15-3, episialin

Gene Name

MUC1

UniProt

P15941

Host

Mouse

Reactivity

Human

Immunogen

Human milk fat globule membranes

Target

Epithelial Membrane Antigen (EMA)

Clonality

Monoclonal

Isotype

IgG1

Clone

EMA 201

Type

Primary Antibody

Applications

IHC, ICC, WB, ELISA

Concentration

1 mg/mL

Format

Purified by Protein A Chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

260-400 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:200

WB Dilution

1:1000

Specificity

No cross-reactivity reported

ICC Dilution

1:200

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Quality Control

1

Trial Size

1

Conjugate

Unconjugated

RRID

AB_3095343

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