Anti-CSPG4/NG2 Antibody FL490 Conjugate

Neuron-glial antigen 2 (NG2) is a chondroitin sulfate proteoglycan that is encoded by the CSPG4 gene in humans. It is an excellent oligodendrocyte marker. NG2 is a hallmark protein of oligodendrocyte progenitor cells (OPCs) and has been linked with familial schizophrenia (de Vrij, F.M., et al, 2018) . NG2 also plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells (Luo, W., et al, 2006) . NG2 localizes at the apical plasma membrane and relocalizes to the lamellipodia of astrocytoma upon PRKCA phosphorylation.

Our Anti-CSPG4/NG2 mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone 6203-34. It detects human, mouse, and rat CSPG4/NG2, and is purified by protein a chromatography. It is great for use in IHC.

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.