What is the catalog number for Anti-CSPG4/NG2 Antibody?

The catalog number for Anti-CSPG4/NG2 Antibody is 77-523.

What sizes are available for Anti-CSPG4/NG2 Antibody?

Anti-CSPG4/NG2 Antibody is available in the following size(s): 100 µL.

Is Anti-CSPG4/NG2 Antibody in stock?

Anti-CSPG4/NG2 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Anti-CSPG4/NG2 Antibody

Our Anti-CSPG4/NG2 mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone 6203-34. It detects human, mouse, and rat CSPG4/NG2, and is purified by Protein A chromatography. It is great for use in IHC, WB.

Product Specifications

Background

Neuron-glial antigen 2 (NG2) is a chondroitin sulfate proteoglycan that is encoded by the CSPG4 gene in humans. It is an excellent oligodendrocyte marker. NG2 is a hallmark protein of oligodendrocyte progenitor cells (OPCs) and has been linked with familial schizophrenia (de Vrij, F.M., et al, 2018) . NG2 also plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells (Luo, W., et al, 2006) . NG2 localizes at the apical plasma membrane and relocalizes to the lamellipodia of astrocytoma upon PRKCA phosphorylation.

Short Description

Our Anti-CSPG4/NG2 mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone 6203-34. It detects human, mouse, and rat CSPG4/NG2, and is purified by protein a chromatography. It is great for use in WB, IHC, ELISA.

Gene Name

CSPG4/NG2

UniProt

Q6UVK1

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids S1583-S2224 of human CSPG4/NG2 (accession number Q6UVK1) produced in CHO cells

Target

CSPG4/NG2

Clonality

Monoclonal

Isotype

IgG1

Clone

6203-34

Type

Primary Antibody

Applications

WB, IHC, ELISA

Field of Research

Glial and Schwann Cell Markers/Astrocyte Markers/Oligodendrocyte Markers

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Molecular Weight

250 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:40

WB Dilution

1:50

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.