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Anti-Centromere Protein Antibody

Our Anti-Centromere proteins human polyclonal primary antibody from Antibodies Incorporated is produced in-house. It detects Centromere proteins, and is purified by Protein A chromatography. It is great for use in ICC.

Product Specifications

Background

Centromere proteins CENPA/B/C are frequent autoantigens in CREST patients

Short Description

Our Anti-Centromere proteins human polyclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone . It detects fish, horse, human, lizard, and mouse Centromere proteins, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Host

Human

Reactivity

Fish, Horse, Human, Lizard, and Mouse

Immunogen

Purified IgG derived from Human anti-Centromere positive serum (also known as CREST patient serum) is useful as a control in ANA diagnostics and also react with other species including mouse, rat, and hamster.

Target

Centromere proteins

Clonality

Polyclonal

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Autoimmune Disease

Concentration

Lot dependent (1-2mg/mL)

Format

Purified by Protein A chromatography

Buffer

1% BSA, 1X PBS pH 7.4, 0.1% sodium azide.

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C.

Notes

CREST patient sera subjected to protein A purification to yield purified IgG

IHC Dilution

1:250

Specificity

No cross-reactivity reported

ICC Dilution

1:250

Quality Control

Product was tested at a series of dilutions by immunocytochemistry on ethanol-fixed Hep2 cells that were in log-phase growth. The staining pattern obtained was consistent with the pattern expected for anti-centromere staining.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2939059

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