What is the catalog number for Anti-GAD65/67 Antibody?

The catalog number for Anti-GAD65/67 Antibody is 75-508.

What sizes are available for Anti-GAD65/67 Antibody?

Anti-GAD65/67 Antibody is available in the following size(s): 100 µL.

Is Anti-GAD65/67 Antibody in stock?

Anti-GAD65/67 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Anti-GAD65/67 Antibody

Our Anti-GAD65/67 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L127/12. It detects human, mouse, and rat GAD65/67, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Product Specifications

Background

There are two forms of the enzyme glutamic-acid decarboxylase (GAD), a 65-kDa form (GAD65) and a 67-kDa form (GAD67) which are the result of the expression of different genes. Further splicing of GAD 67 produces one or two smaller variants, including GAD25 and GAD44. GAD67 and GAD65 are expressed in adult brain and the pancreas. The truncated forms GAD25 and GAD44 are expressed during embryonic development. Glutamic acid decarboxylase enzyme functions to catalyze the production of gamma-aminobutyric acid from L-glutamic acid.

Short Description

Our Anti-GAD65/67 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L127/12. It detects human, mouse, and rat GAD65/67, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA, AT.

Synonyms

GAD67, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD1, GAD and DCE1

Gene Name

GAD1, GAD, GAD67

UniProt

Q99259

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1-594 (full-length) of human GAD67 (accession number Q99259) produced recombinantly in E. Coli

Target

GAD65/67

Clonality

Monoclonal

Isotype

IgG2a

Clone

L127/12

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA, AT

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

70 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Cross-reacts with GAD25, GAD44 and GAD65/GAD2

ICC Dilution

1:100

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.