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Anti-GAD67 Antibody (BSA and Azide free)

Our carrier-free Anti-GAD67 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L127/8. It detects human, mouse, and rat GAD67, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Product Specifications

Background

Glutamate decarboxylase 1, GAD67 or GAD1 is a member of the group II decarboxylase family and catalyzes the production of GABA from L-glutamic acid. GAD67 is expressed in brain (throughout the cell), in insulin producing ßcells of the pancreas, and several non-neuronal tissues during development. Alterations in GAD67 have been shown to lead to pyridoxine dependency with seizures. Other disorders associated with GAD67 are Cerebral Palsy, Spastic Quadriplegic, 1 and Inherited Congenital Spastic Tetraplegia.

Short Description

Our Anti-GAD67 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L127/8. It detects human, mouse, and rat GAD67, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

GAD67, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD1, GAD and DCE1

Gene Name

GAD1, GAD, GAD67

UniProt

Q99259

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1-594 (full-length) of human GAD67 (accession number Q99259) produced recombinantly in E. Coli

Target

GAD67

Clonality

Monoclonal

Isotype

IgG2b

Clone

L127/8

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS

Molecular Weight

70 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and buffer exchanged into 1X PBS. Purified mAbs are >90% specific antibody.

Specificity

Cross-reacts with GAD25 Does not cross-react with GAD44 or GAD65/GAD2

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Conjugate

Unconjugated

Frequently Asked Questions

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