Anti-Synapsin-1/3 Antibody FL650 Conjugate

The synapsin family of proteins are neuron specific phospho-proteins comprised of synapsins I, II and III. Synapsins I and II are abundantly expressed pre-synaptic proteins that play key roles in synaptic plasticity and synaptogenesis (Feng et al., 2002; Nayak et al., 1996) . Synapsin III is the most recent member of the synapsin family to be indentified and is primarily expressed in extra-synaptic sites such as cell bodies and growth cones (Ferreira A. et al., 2000) . Synapsin III is expressed at much lower levels than synapsin I and II in the adult brain and is therefore thought to be involved in early neural development (Kao HT, et al., 1998) .

Our Anti-Synapsin-1/3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L125/129. It detects human, mouse, and rat Synapsin-1/3, and is purified by protein a chromatography. It is great for use in IHC.

Fusion protein amino acids 76-417 (actin-binding and synaptic-vesicle binding domains) of human Synapsin-III (accession number O14994) produced recombinantly in E. Coli

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.