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Anti-VAPA Antibody FL550 Conjugate

Selected Variant

75-495-FL550

Size: 200 µL

Our Anti-VAPA mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N479/22. It is KO validated, detects mouse and rat VAPA, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

VAPA and VAPB are members of the VAMP-associated protein family (VAP) (aka vesicle-associated membrane proteins) which are abundant scaffolding proteins predominantly localized to the endoplasmic reticulum (ER) . VAPs have recently been shown to interact with Kv2.1 and Kv2.2 channels forming junctions to link the ER to the plasma membrane (Johnson B. et al., 2018) .

Short Description

Our Anti-VAPA mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N479/22. It is KO validated, detects mouse and rat VAPA, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

VAPA (Vesicle-associated membrane protein-associated protein A) (VAMP-associated protein A) (33 kDa VAMPassociatedprotein) (VAMP-A) (VAP-33)

Gene Name

Vapa Vap33

UniProt

Q9Z270

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 1-219 (N-terminus) of rat VAPA (accession number Q9Z270) produced recombinantly in E. Coli

Target

VAPA

Clonality

Monoclonal

Isotype

IgG2a

Clone

N479/22

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Vesicle Trafficking/Fusion

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

30 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

ICC Quality Control

1

Conjugate

FL550 Ex: 550 nm, Em: 575 nm

RRID

AB_2940649

Price

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