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Anti-Bassoon Antibody

Our Anti-Bassoon mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L124/59. It detects mouse and rat Bassoon, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Product Specifications

Background

Bassoon is a large (420-kDa) scaffolding protein specifically localized at the active zone of presynaptic nerve terminals. Bassoon is involved in the development of presynaptic terminals and in the regulation of neurotransmitter release at both excitatory and inhibitory brain synapses (Richter K. et al., 1999) .

Short Description

Our Anti-Bassoon mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L124/59. It detects mouse and rat Bassoon, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA, AT.

Synonyms

Protein bassoon

Gene Name

Bsn

UniProt

O88778

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (accession number O88778) produced recombinantly in E. Coli

Target

Bassoon

Clonality

Monoclonal

Isotype

IgG2b

Clone

L124/59

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA, AT

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

400 kDa for full-length protein, many smaller bands possible depending on sample used

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:500

Specificity

No cross-reactivity reported

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

Trial Size

1

Conjugate

Unconjugated

IHC Quality Control

1

RRID

AB_2744564

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