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Anti-Calretinin Antibody

Our Anti-Calretinin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L122/6. It detects human, mouse, and rat Calretinin, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996) . Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000) .

Short Description

Our Anti-Calretinin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L122/6. It detects human, mouse, and rat Calretinin, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

CALB2 (Calretinin) (29 kDa calbindin)

Gene Name

CALB2 CAB29

UniProt

P22676

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1-178 (N-terminus) of human CALB2 (accession number) produced recombinantly in E. Coli

Target

Calretinin

Clonality

Monoclonal

Isotype

IgG2b

Clone

L122/6

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

GABAergic Neuronal Marker/Cell Signaling

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

25-35 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2797368

Frequently Asked Questions

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