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Anti-VSP/TPTE Antibody

Our Anti-VSP/TPTE mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N432/21. It detects chicken, Xenopus, zebrafish VSP/TPTE, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

TPTEs (transmembrane phosphatases with tensin homology) aka VSPs (voltage sensing phosphatases) catalyze the dephosphorylation of phosphatidylinositol phosphates (PIPs) that regulate diverse aspects of cell membrane physiology including cell division, growth and migration (Ratzan W. et al., 2019) . TPTEs are also thought to be involved in signal transduction pathways of the endocrine or spermatogenetic function of the testis (Chen H. et al., 1999) .

Short Description

Our Anti-VSP/TPTE mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N432/21. It detects chicken, xenopus, zebrafish VSP/TPTE, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Transmembranephosphatase with tensin homology (voltage-sensing phosphoinositide phosphatase) (VSP)

Gene Name

TPTE

UniProt

Q4V9E4

Host

Mouse

Reactivity

Chicken, Xenopus, Zebrafish

Immunogen

Fusion protein amino acids 153-335 of zebrafish TPTE (accession number Q4V9E4) produced recombinantly in E. Coli

Target

VSP/TPTE

Clonality

Monoclonal

Isotype

IgG2a

Clone

N432/21

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Cell Signaling

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

40-60 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

WB Dilution

1:500

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Zebrafish

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2744560

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