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Anti-nNOS/NOS1 Antibody

Our Anti-nNOS/NOS1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L121/25. It detects human, mouse, and rat nNOS/NOS1, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Product Specifications

Background

Nitric Oxide Synthase 1 is encoded by the gene NOS1. NOS1 is a member of the NOS family. NOS1 synthesizes nitric oxide from L-arginine, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. NOS1 is expressed ubiquitously, with high levels of expression in skeletal muscle. Diseases associated with NOS1 include Achalasia, Familial Esophageal and Pyloric Stenosis, Infantile Hypertrophic, 1.

Short Description

Our Anti-nNOS/NOS1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L121/25. It detects human, mouse, and rat nNOS/NOS1, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA, AT.

Synonyms

NOS1 (Brain nitric oxide synthase) (Constitutive NOS) (Neuronal NOS) (Peptidyl-cysteine S-nitrosylase NOS1)

Gene Name

NOS1

UniProt

P29475

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 350-720 of human NOS1 (accession number P29475) produced recombinantly in E. Coli

Target

NNOS/NOS1

Clonality

Monoclonal

Isotype

IgG2b

Clone

L121/25

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA, AT

Field of Research

Cell Signaling

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

155-165 kDa for full-length protein, smaller fragments possible due to proteolysis and/or variable sample preparation

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2725813

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