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Anti-Collybistin Antibody

Our Anti-Collybistin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L120/12. It detects mouse and rat Collybistin, and is purified by Protein A chromatography. It is great for use in AT, ICC, WB.

Product Specifications

Background

Rho guanine nucleotide exchange factor 9 or Collybistin is encoded by the gene ARHGEF9. Collybistin is a guanine nucleotide exchange factor (GEF) and is a member of the Rho-like GTPase family. Collybistin functions to cycle active GTP to inactive GDP. Collybistin is involved in microfilament regulation and clustering of some GABA receptors and It is expressed in brain at postsynaptic membranes. Diseases associated with ARHGEF9 include Early Infantile Epileptic Encephalopathy and Hyperekplexia.

Short Description

Our Anti-Collybistin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L120/12. It detects mouse and rat Collybistin, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA, AT.

Synonyms

Collybistin (Rho guanine nucleotide exchange factor 9) (Rac/Cdc42 guanine nucleotide exchange factor 9)

Gene Name

Arhgef9

UniProt

Q9QX73

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 105-455 of rat Collybistin (accession number Q9QX73) produced recombinantly in E. Coli

Target

Collybistin

Clonality

Monoclonal

Isotype

IgG1

Clone

L120/12

Type

Primary Antibody

Applications

WB, ICC, ELISA, AT

Field of Research

Cell Signaling/G Protein Signaling

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

50-60 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

WB Dilution

1:5000

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2728741

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