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Anti-BDNF Antibody

Our Anti-BDNF mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N450/53. It detects mouse and rat BDNF, and is purified by Protein A chromatography. It is great for use in IHC, WB.

Product Specifications

Background

Brain-derived neurotrophic factor is encoded by the gene BDNF and is a member of the neurotrophin family of growth factors. BDNF is a secreted signaling molecule with may important functions including axonal growth, modulation of dendritic growth, the promotion of survival/differentiation of neuronal populations in peripheral and central nervous systems, regulation of synaptic transmission/plasticity and long-term potentiation. BDNF is expressed highly in brain (in the hippocampus, amygdala, cerebral cortex and cerebellum) and expressed in lung, heart and skeletal muscle. Diseases associated with this gene include Congenital Central Hypoventilation Syndrome, Wilms Tumor, Aniridia and Mental Retardation Syndrome.

Short Description

Our Anti-BDNF mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N450/53. It detects mouse and rat BDNF, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Brain-derived neurotrophic factor (Abrineurin)

Gene Name

Bdnf

UniProt

P21237

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 1-249 (full length) of mouse BDNF (accession number P21237) produced recombinantly in E. Coli

Target

BDNF

Clonality

Monoclonal

Isotype

IgG2b

Clone

N450/53

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Neurogenesis and Development

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

25-40 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2722670

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