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Anti-VGAT Antibody (BSA and Azide free)

Our carrier-free Anti-VGAT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L118/80. It detects mouse and rat VGAT, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997) . The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998) . Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005) .

Short Description

Our Anti-VGAT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L118/80. It detects mouse and rat VGAT, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Vesicular inhibitory amino acid transporter (GABA and glycine transporter) (Solute carrier family 32 member 1) (Vesicular GABA transporter) (mVGAT) (mVIAAT)

Gene Name

Slc32a1 Vgat Viaat

UniProt

O35633

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 1-133 (cytoplasmic N-terminus) of mouse VGAT (accession number O35633) produced recombinantly in E. Coli

Target

VGAT

Clonality

Monoclonal

Isotype

IgG2a

Clone

L118/80

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Transporter Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS

Molecular Weight

50 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and buffer exchanged into 1X PBS. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

Unconjugated

Frequently Asked Questions

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