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Anti-Parvalbumin Antibody

Our Anti-Parvalbumin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L114/3. It detects mouse and rat Parvalbumin, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Parvalbumin (PA) is a classical small, mostly cytosolic Ca2+-binding protein of the EF-hand superfamily expressed in vertebrates in a tissue- and cell-specific manner, serving as a magnesium/ calcium buffer (Permyakov EA et al., 2017) . Parvalbumin (PV) -expressing interneurons are a class of inhibitory neurons that fire rapid barrages of action potentials, and are accordingly named “fast-spiking” interneurons (Trachtenberg J. 2015) .

Short Description

Our Anti-Parvalbumin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L114/3. It detects mouse and rat Parvalbumin, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Parvalbumin alpha

Gene Name

Pvalb Pva

UniProt

P02625

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 1-110 (full-length) of rat Parvalbumin (accession number P02625) produced recombinantly in E. Coli

Target

Parvalbumin

Clonality

Monoclonal

Isotype

IgG2a

Clone

L114/3

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

20 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:500

Specificity

No cross-reactivity reported

ICC Dilution

1:1000

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2651167

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