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Anti-CALB1/calbindin Antibody (BSA and Azide free)

Our carrier-free Anti-CALB1/calbindin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L109/57. It detects human, mouse, and rat CALB1/calbindin, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Product Specifications

Background

Calbindin (aka. calbindin D28k) is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is predominantly expressed in certain types of neurons, particularly in dendrites and perikarya of the cerebellum and is thought to buffer entry of calcium upon stimulation of glutamate receptors (Andressen et al., 1993) . Calbindin has recently been shown to play a critical role in mitochondrial dysfunction and loss of synaptic proteins in vivo in an Alzheimer’s disease mouse model (Kook et al., 2014) .

Short Description

Our Anti-CALB1/calbindin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L109/57. It detects human, mouse, and rat CALB1/calbindin, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Calbindin (Calbindin D28) (D-28K) (Vitamin D-dependent calcium-binding protein, avian-type)

Gene Name

CALB1 CAB27

UniProt

P05937

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1-261 (full-length) of human CALB1 (accession number P05937) produced recombinantly in E. Coli

Target

CALB1/calbindin

Clonality

Monoclonal

Isotype

IgG1

Clone

L109/57

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS

Molecular Weight

30 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and buffer exchanged into 1X PBS. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Conjugate

Unconjugated

Frequently Asked Questions

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