What is the catalog number for Anti-GABA-A-R-Gamma2L Antibody?

The catalog number for Anti-GABA-A-R-Gamma2L Antibody is 75-442.

What sizes are available for Anti-GABA-A-R-Gamma2L Antibody?

Anti-GABA-A-R-Gamma2L Antibody is available in the following size(s): 100 µL.

Is Anti-GABA-A-R-Gamma2L Antibody in stock?

Anti-GABA-A-R-Gamma2L Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

Anti-GABA-A-R-Gamma2L Antibody

Our Anti-GABA-A-R-Gamma2L mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N452/69. It detects human, mouse, and rat GABA-A-R-Gamma2L, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Gamma-aminobutyric acid receptor subunit gamma-2, or GABA AR gamma2 is encoded by the gene GABRG2. GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR gamma2 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR gamma2 has been found expressed in stomach and bladder. In brain, GABA AR gamma2 is expressed at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. Mutations in this gene have been associated with epilepsy and febrile seizures. Note that GABA AR γ2 subunit has two forms, designated “short” (γ2S) and “long” (γ2L) that differ only in eight amino acids inserted into the large intracellular loop of the subunit (Whiting et al., 1990) .

Short Description

Our Anti-GABA-A-R-Gamma2L mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N452/69. It detects human, mouse, and rat GABA-A-R-Gamma2L, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Synonyms

Gamma-aminobutyric acid receptor subunit gamma-2 (GABA (A) receptor subunit gamma-2)

Gene Name

GABRG2

UniProt

P18507-2

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 360-445 (cytoplasmic C-terminus) of human GABA-A-R-Gamma2L (accession number P18507-2) produced recombinantly in E. Coli

Target

GABA-A-R-Gamma2L

Clonality

Monoclonal

Isotype

IgG2a

Clone

N452/69

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Neurotransmitter Receptors

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

45 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

WB Dilution

1:1000

IP Dilution

4 ug

Specificity

Does not cross-react with GABA-A-R-Gamma2S, GABA-A-R-Gamma1 or GABAA-A-R-Gamma3

ICC Dilution

1:1000

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.