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Anti-Slc18a2/VMAT2 Antibody

Our Anti-Slc18a2/VMAT2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N449/73. It detects human, mouse, and rat Slc18a2/VMAT2, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Product Specifications

Background

Vesicular neurotransmitter transporters sequester the transmitters into synaptic vesicles (Erickson et al., 1996) . The vesicular monoamine transporter 2 (VMAT2) is responsible for catecholamine and serotonin storage in central synapses. Antibodies specific for VMAT have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005) .

Short Description

Our Anti-Slc18a2/VMAT2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N449/73. It detects human, mouse, and rat Slc18a2/VMAT2, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Synonyms

Slc18a2 protein

Gene Name

Slc18a2

UniProt

Q66L40

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 42-132 (first lumenal domain) of mouse Slc18a2 (accession number Q66L40) produced recombinantly in E. Coli

Target

Slc18a2/VMAT2

Clonality

Monoclonal

Isotype

IgG2b

Clone

N449/73

Type

Primary Antibody

Applications

WB, ICC, ELISA

Field of Research

Transporter Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

60 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Slc18a1/VMAT1 or Slc18a3/VAChT

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2686911

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