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Anti-VAChT Antibody

Our Anti-VAChT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N425/45. It detects mouse and rat VAChT, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Vesicular acetylcholine transporter (VaCht), or Solute Carrier Family 18 Member A3 is encoded by the gene Slc18a3 and is a member of the vesicular amine transporter family. VaCht transports acetylcholine into secretory vesicles for release into the extracellular space. VaCht is expressed in major cholinergic cell groups in the peripheral and central cholinergic nervous systems. Diseases associated with SLC18A3 include Myasthenic Syndrome and Presynaptic and Presynaptic Congenital Myasthenic Syndromes.

Short Description

Our Anti-VAChT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N425/45. It detects mouse and rat VAChT, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Vesicular acetylcholine transporter (VAChT) (rVAT) (Solute carrier family 18 member 3)

Gene Name

Slc18a3 Vacht Vat

UniProt

Q62666

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 55-125 (first vesicle lumen domain) of rat VAChT (accession number Q62666) produced recombinantly in E. Coli

Target

VAChT

Clonality

Monoclonal

Isotype

IgG1

Clone

N425/45

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Transporter Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

60 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with VMAT1 or VMAT2

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

Conjugate

Unconjugated

IHC Quality Control

1

RRID

AB_2632411

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