Anti-Glycine Receptor Alpha3 Antibody

Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003) . The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000) . The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997) . These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001) . The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002) .

Our Anti-Glycine receptor Alpha3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N424/45. It detects human, mouse, and rat Glycine receptor Alpha3, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.