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Anti-GFP (Green Fluorescent Protein) Antibody

Our Anti-GFP (green fluorescent protein) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N86/44. It detects GFP (green fluorescent protein), and is purified by Protein A chromatography. It is great for use in ICC, WB.

Product Specifications

Background

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Short Description

Our Anti-GFP (green fluorescent protein) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N86/44. It detects n/a GFP (green fluorescent protein), and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Synonyms

Green fluorescent protein

Gene Name

GFP

UniProt

P42212

Host

Mouse

Immunogen

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Target

GFP (green fluorescent protein)

Clonality

Monoclonal

Isotype

IgG2b

Clone

N86/44

Type

Primary Antibody

Applications

WB, ICC, ELISA

Field of Research

Anti-Tag Antibodies

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

30 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

WB Dilution

1:1000

Specificity

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively) Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Jellyfish

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2532048

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