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Anti-ANO5/TMEM16E Antibody

Our Anti-ANO5/TMEM16E mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N421A/85. It detects human ANO5/TMEM16E, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Product Specifications

Background

Anoctamin-5, Transmembrane Protein 16E or -ANO5/TMEM16E is encoded by the gene ANO5. ANO5 belongs to the anoctamin family of membrane proteins many of which are calcium-activated chloride channels. ANO5 does not exhibit this channel activity. ANO5 is expressed in brain, heart, kidney, lung, skeletal muscle as well as other tissues. Diseases associated with ANO5 include Gnathodiaphyseal Dysplasia and Miyoshi Muscular Dystrophy 3.

Short Description

Our Anti-ANO5/TMEM16E mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N421A/85. It detects human and mouse ANO5/TMEM16E, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Anoctamin-5 (Gnathodiaphyseal dysplasia 1 protein) (Transmembrane protein 16E)

Gene Name

ANO5 GDD1 TMEM16E

UniProt

Q75V66

Host

Mouse

Reactivity

Human and Mouse

Immunogen

Fusion protein amino acids 1-299 (cytoplasmic N-terminus) of human ANO5 (accession number Q75V66) produced recombinantly in E. Coli

Target

ANO5/TMEM16E

Clonality

Monoclonal

Isotype

IgG1

Clone

N421A/85

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Ion Channels and Modulators/Other Ion Channels

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

110 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

WB Dilution

1:1000

Specificity

Does not cross-react with ANO6/TMEM16F

ICC Dilution

1:100

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2531893

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