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Anti-NCKX4 Antibody

Our Anti-NCKX4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N414/25. It is KO validated, detects human and rat NCKX4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Sodium/potassium/calcium exchanger 4, Na (+) /K (+) /Ca (2+) -Exchange Protein 4 or NCKX4 is encoded by the gene SLC24A4. NCKX4 is a member of the potassium-dependent sodium/calcium exchanger protein family. NCKX4 is thought to play roles in olfactory function and in tooth enamel formation. It is expressed in the aorta, lung, thymus and brain, particularly enriched in neurons. Diseases associated with SLC24A4 include cause amelogenesis imperfecta.

Short Description

Our Anti-NCKX4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N414/25. It is KO validated, detects human, mouse, rat, and zebrafish NCKX4, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Sodium/potassium/calcium exchanger 4 (Na (+) /K (+) /Ca (2+) -exchange protein 4) (Solute carrier family 24 member 4)

Gene Name

SLC24A4 NCKX4

UniProt

Q8NFF2-3

Host

Mouse

Reactivity

Human, Mouse, Rat, and Zebrafish

Immunogen

Fusion protein amino acids 246-424 (third intracellular loop) of human NCKX4 isoform 3 (accession number Q8NFF2-3) produced recombinantly in E. Coli

Target

NCKX4

Clonality

Monoclonal

Isotype

IgG2b

Clone

N414/25

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Transporter Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

60 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:500

WB Dilution

1:500

Specificity

Does not cross-react with NCKX2 or NCKX3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2491110

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