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Anti-Kv2.2 Potassium Channel Antibody

Our Anti-Kv2.2 potassium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N372B/1. It is KO validated, detects human, mouse, and rat Kv2.2 potassium channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999) . Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK) channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003) . Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996) .

Short Description

Our Anti-Kv2.2 potassium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N372B/1. It is KO validated, detects human, mouse, and rat Kv2.2 potassium channel, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Potassium voltage-gated channel subfamily B member 2 (CDRK) (Voltage-gated potassium channel subunit Kv2.2)

Gene Name

Kcnb2

UniProt

Q63099

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 717-907 (cytoplasmic C-terminus) of rat Kv2.2 long isoform (accession number Q63099) produced recombinantly in E. Coli

Target

Kv2.2 potassium channel

Clonality

Monoclonal

Isotype

IgG1

Clone

N372B/1

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Ion Channels and Modulators/K+ Channels

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

120 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Kv2.2 short isoformDoes not cross-react with Kv2.1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2315870

Frequently Asked Questions

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