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Anti-Kv3.3 Potassium Channel Antibody

Our Anti-Kv3.3 potassium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N375/67. It detects human, mouse, and rat Kv3.3 potassium channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Potassium voltage-gated channel subfamily C member 3 or Kv3.3 potassium channel, is a member of the potassium channel, voltage-gated, shaker-related subfamily and the Shaw subfamily (the family includes 4 members Kv3.1-Kv3.4) . Note that this protein has several splice variants and that antibody N375/67 recognizes both Kv3.3b and Kv3.3a. Kv3.3 is primarily expressed in brain. Kv3.3 is highly expressed in purkinje cells and deep cerebellar nuclei in the cerebellum. Kv3.3a is also expressed in brain, but not found in the cerebellum. Kv3.3 is a membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Diseases associated with the gene for this protein (Kcnc3) include Spinocerebellar Ataxia 13 and Autosomal recessive ataxic cerebral palsy.

Short Description

Our Anti-Kv3.3 potassium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N375/67. It detects human, mouse, and rat Kv3.3 potassium channel, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Potassium voltage-gated channel subfamily C member 3 (KSHIIID) (Voltage-gated potassium channel subunit Kv3.3)

Gene Name

Kcnc3

UniProt

Q63959

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 628-728 (cytoplasmic C-terminus) of mouse Kv3.3b (accession number Q63959) produced recombinantly in E. Coli

Target

Kv3.3 potassium channel

Clonality

Monoclonal

Isotype

IgG1

Clone

N375/67

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Ion Channels and Modulators/K+ Channels

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

80-100 kDa (depending on isoform)

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Kv3.4 or Kv3.2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2315872

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