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Anti-BAF53a Antibody FL650 Conjugate

Our Anti-BAF53a mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N336B/83. It detects human, mouse, and rat BAF53a, and is purified by Protein A chromatography. It is great for use in ICC.

Product Specifications

Background

Actin-like protein 6A, or BAF Complex 53 KDa Subunit (BAF53a) is encoded by the gene ACTL6A and is a member of actin-related proteins family. It is a subunit of the neuron specific chromatin remodeling complex (nBAF complex) . BAF53a is thought to be involved in transcriptional activation and repression of chromatin remodeling. BAF53a is a subunit of the neural progenitors-specific chromatin remodeling complex (npBAF complex) . BAF53a is expressed in many different cell types. Diseases associated with ACTL6A include Coffin-siris syndrome and Brachymorphism-Onychodysplasia-Dysphalangism Syndrome.

Short Description

Our Anti-BAF53a mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N336B/83. It detects human, mouse, and rat BAF53a, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Actin-like protein 6A (53 kDa BRG1-associated factor A) (Actin-related protein Baf53a) (ArpNbeta) (BRG1-associated factor 53A) (BAF53A) (INO80 complex subunit K)

Gene Name

ACTL6A BAF53 BAF53A INO80K

UniProt

O96019

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 43-119 (MVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWDSFQAILDHTYKMHVKSEASLHP, actin subdomain 2) of human BAF53a (accession number O96019) produced recombinantly in E. Coli

Target

BAF53a

Clonality

Monoclonal

Isotype

IgG2b

Clone

N336B/83

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Neurogenesis and Development/Transcription Regulation

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

53 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with BAF53b

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

ICC Quality Control

1

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

RRID

AB_2940147

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