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Anti-NSD3 Antibody FL650 Conjugate

Our Anti-NSD3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N348/82. It detects human, mouse, and rat NSD3, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Nuclear Receptor Binding SET Domain Protein 3 is encoded by the gene NSD3. NSD3 is a member of the class V-like SAM-binding methyltransferase superfamily, histone/lysine methlytransferase family, SET2 subfamily. NSD3 methylates histone H3 at lysine residues 4 and 27, which represses gene expression. NSD3 is expressed in the brain, heart, skeletal muscle, liver, and lungs. Diseases associated with NSD3 include Wolf-Hirschhorn Syndrome and Nut Midline Carcinoma.

Short Description

Our Anti-NSD3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N348/82. It detects human, mouse, and rat NSD3, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Histone-lysine N-methyltransferase NSD3 (EC 2.1.1.43) (Nuclear SET domain-containing protein 3) (Protein whistle) (WHSC1-like 1 isoform 9 with methyltransferase activity to lysine) (Wolf-Hirschhorn syndrome candidate 1-like protein 1) (WHSC1-like protein 1)

Gene Name

NSD3 WHSC1L1 DC28

UniProt

Q9BZ95

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 21-251 (N-terminus) of human NSD3 (accession number Q9BZ95) produced recombinantly in E. Coli

Target

NSD3

Clonality

Monoclonal

Isotype

IgG1

Clone

N348/82

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Epigenetics

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

>160 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

IHC Quality Control

1

RRID

AB_2940123

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