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Anti-AMIGO-1 Antibody

Our Anti-AMIGO-1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L86/33. It is KO validated, detects human, mouse, and rat AMIGO-1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Amphoterin-induced protein 1, amphoterin-induced gene and open reading frame, Adhesion Molecule With Ig Like Domain 1 or Amigo-1 is encoded by the gene AMIGO1 and is a member of the AMIGO family. Amigo1 acts as a neuronal adhesion protein and contains leucine-rich repeat and immunoglobin domains. It is thought to play a role in cell to cell interactions and in neuronal plasticity/regenereation. Amigo1 is expressed in the nervous system and at low levels in liver, kidney, spleen, lung and heart.

Short Description

Our Anti-AMIGO-1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L86/33. It is KO validated, detects human, mouse, and rat AMIGO-1, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Amphoterin-induced protein 1 (AMIGO-1) (Alivin-2)

Gene Name

Amigo1 Ali2 Amigo Kiaa1163

UniProt

Q80ZD8

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1 (accession number Q86WK6) produced recombinantly in E. Coli

Target

AMIGO-1

Clonality

Monoclonal

Isotype

IgG2a

Clone

L86/33

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Ion Channels and Modulators/Ion Channel Modulators

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

60-80 kDa depending on maturity/glycosylation

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2315765

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