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Anti-Lgi1 Antibody FL490 Conjugate

Our Anti-Lgi1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N283/7. It is KO validated, detects human, mouse, and rat Lgi1, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Product Specifications

Background

Leucine Rich Glioma Inactivated 1 is encoded by the gene LGI1. LGI1 is a member of the secreted leucine-rich repeat (LRR) superfamily. LGI1 may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. LGI1 is expressed in the brain. Diseases associated with LGI1 include Epilepsy, Familial Temporal Lobe, 1 and Autosomal Dominant Epilepsy with Auditory Features.

Short Description

Our Anti-Lgi1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N283/7. It is KO validated, detects human, mouse, and rat Lgi1, and is purified by protein a chromatography. It is great for use in IHC, ICC.

Synonyms

Leucine-rich glioma-inactivated protein 1

Gene Name

Lgi1

UniProt

Q9JIA1

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse Lgi1 (accession number Q9JIA1) produced recombinantly in E. Coli

Target

Lgi1

Clonality

Monoclonal

Isotype

IgG2a

Clone

N283/7

Type

Primary Antibody

Applications

IHC, ICC

Field of Research

Ion Channels and Modulators/Ion Channel Modulators

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

60 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Lgi2, Lgi3 or Lgi4 (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

FL490 Ex: 491 nm, Em: 515 nm

RRID

AB_2940072

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