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Anti-Synaptotagmin-5 Antibody

Our Anti-Synaptotagmin-5 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N274/8. It detects mouse and rat Synaptotagmin-5, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Along with synaptotagmins 1 and 2, synaptotagmin 5 (also known as synaptotagmin 9) has been shown to function as a Ca (2+) sensor for fast synchronous neurotransmitter release (Xu J et al., 2007) .

Short Description

Our Anti-Synaptotagmin-5 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N274/8. It detects mouse and rat Synaptotagmin-5, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Synaptotagmin-9 (Synaptotagmin IX) (SytIX) (Synaptotagmin V)

Gene Name

Syt9 Syt5

UniProt

Q9R0N9

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 236-323 (cytoplasmic C2A domain) of mouse Synaptotagmin-5 (accession number Q9R0N9) produced recombinantly in E. Coli

Target

Synaptotagmin-5

Clonality

Monoclonal

Isotype

IgG1

Clone

N274/8

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Vesicle Trafficking/Fusion

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

45 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Synaptotagmin-10 (or other Synaptotagmins)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2315936

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