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Anti-Synaptotagmin-12 Antibody

Our Anti-Synaptotagmin-12 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N277/7. It detects mouse and rat Synaptotagmin-12, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 12 differs from classical synaptotagmins in that it does not bind Ca (2+) . In synaptic vesicles, synaptotagmin-12 forms a complex with synaptotagmin-1 that prevents synaptotagmin-1 from interacting with SNARE complexes (Maximov A. et al., 2007) .

Short Description

Our Anti-Synaptotagmin-12 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N277/7. It detects mouse and rat Synaptotagmin-12, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Synaptotagmin-12 (Synaptotagmin XII) (SytXII)

Gene Name

Syt12

UniProt

Q920N7

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 168-255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12 (also known as Syt12, Synaptotagmin XII, SytXII, Synaptotagmin-related gene 1 protein, Srg1 and Sytr1, accession number Q920N7) produced recombinantly in E. Coli

Target

Synaptotagmin-12

Clonality

Monoclonal

Isotype

IgG1

Clone

N277/7

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Vesicle Trafficking/Fusion

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

45 kDa (plus others)

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Synaptotagmin-6 (or other Synaptotagmins)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2315940

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