Anti-Notch1 Antibody

Our Anti-Notch1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N253/32. It detects mouse and rat Notch1, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Product Specifications

Background

Notch Receptor 1 is encoded by the gene NOTCH1. NOTCH1 is a member of the NOTCH family. NOTCH1 is involved in processes related to cell fate specification, differentiation, proliferation, and survival. NOTCH1 is highly expressed in the brain, lungs, and thymus. Diseases associated with NOTCH1 include Aortic Valve Disease 1 and Adams-Oliver Syndrome 5.

Short Description

Our Anti-Notch1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N253/32. It detects mouse and rat Notch1, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Synonyms

Neurogenic locus notch homolog protein 1 (Notch 1) (Motch A) (mT14) (p300) [Cleaved into: Notch 1 extracellular truncation (NEXT) ; Notch 1 intracellular domain (NICD) ]

Gene Name

Notch1 Motch

UniProt

Q01705

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 (accession number Q01705) produced recombinantly in E. Coli

Target

Notch1

Clonality

Monoclonal

Isotype

IgG1

Clone

N253/32

Type

Primary Antibody

Applications

WB, ICC, ELISA

Field of Research

Neurogenesis and Development

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

>270 kDa, ~120 kDa and smaller fragments (due to proteolysis)

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with Notch2 or Notch3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.