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Anti-NSD1 Antibody FL490 Conjugate

Our Anti-NSD1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N312/10. It detects human and mouse NSD1, and is purified by Protein A chromatography. It is great for use in ICC.

Product Specifications

Background

Nuclear Receptor Binding SET Domain Protein 1 is encoded by the gene NSD1. NSD1 is a member of the class V-like SAM-binding methyltransferase superfamily. NSD1 is a histone methyltransferase that is capable of both negatively or positively influencing transcription, depending on the cellular context. NSD1 is expressed in the brain, kidney, spleen, skeletal muscle, thymus, and lung. Diseases associated with NSD1 include Sotos Syndrome 1 and Overgrowth Syndrome.

Short Description

Our Anti-NSD1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N312/10. It detects human and mouse NSD1, and is purified by protein a chromatography. It is great for use in ICC.

Synonyms

Histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific (EC 2.1.1.43) (Androgen receptor coactivator 267 kDa protein) (Androgen receptor-associated protein of 267 kDa) (H3-K36-HMTase) (H4-K20-HMTase) (Lysine N-methyltransferase 3B) (Nuclear receptor-binding SET domain-containing protein 1) (NR-binding SET domain-containing protein)

Gene Name

NSD1 ARA267 KMT3B

UniProt

Q96L73

Host

Mouse

Reactivity

Human and Mouse

Immunogen

Fusion protein amino acids 2129-2696 (C-terminus) of human NSD1 (accession number Q96L73) produced recombinantly in E. Coli

Target

NSD1

Clonality

Monoclonal

Isotype

IgG2b

Clone

N312/10

Type

Primary Antibody

Applications

ICC

Field of Research

Epigenetics

Concentration

0.5 mg/mL

Format

Purified by Protein A chromatography

Buffer

PBS with 0.09% azide

Molecular Weight

300 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with NSD2 or NSD3

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Human

ICC Quality Control

1

Conjugate

FL490 Ex: 491 nm, Em: 515 nm

RRID

AB_2939996

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