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Anti-Synaptotagmin-3 Antibody

Our Anti-Synaptotagmin-3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N278/19. It detects human, mouse, and rat Synaptotagmin-3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 3 is predominantly expressed in postsynaptic endocytic zones of neurons and has recently been shown to internalize AMPA receptors to depress synaptic strength and promote forgetting (Awasthi A. et al., 2019) .

Short Description

Our Anti-Synaptotagmin-3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N278/19. It detects human, mouse, and rat Synaptotagmin-3, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Synaptotagmin-3 (Synaptotagmin III) (SytIII)

Gene Name

Syt3

UniProt

O35681

Host

Mouse

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 442-533 (cytoplasmic C2B domain) of mouse Synaptotagmin-3 (accession number O35681) produced recombinantly in E. Coli

Target

Synaptotagmin-3

Clonality

Monoclonal

Isotype

IgG1

Clone

N278/19

Type

Primary Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Vesicle Trafficking/Fusion

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

75 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with other Synaptotagmins

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_11001556

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